Confocal Microscopy

Confocal microscopy, most frequently called confocal laser scanning microscopy (CLSM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph. This is done by means of using a spatial pinhole to block out-of-focus light in image formation. In food products this technique allows imaging of the distribution of ingredients (e.g. protein, fat, carbohydrates) in a product. This in turn can be used to gain insight in the functionality of ingredients or the effects of processing steps on food products.